RESUMO
Characteristics of new Bacillus subtilis isolates from the environment and had high antagonistic activity were studied. Absence of virulence and toxin production allows to regard them as potential probiotic cultures. Growth media for cultivation of B.subtilis were chosen according to the principle of individual adequacy in system, which give the ability to optimize processes of cultivation, obtaining of inhibitory substances and antagonistic activity spectrum determination.
Assuntos
Bacillus subtilis/fisiologia , Antibiose , Biomassa , Meios de Cultura , Microbiologia Ambiental , Bactérias Gram-Negativas/fisiologia , Bactérias Gram-Positivas/fisiologia , ProbióticosRESUMO
Study showed that synthesis of specific IgG occurs in rabbits immunized with recombinant outer membrane protein F (OprF) of Pseudomonas aeruginosa and that these antibodies inhibit grow of P. aeruginosa in vitro. In vivo studies on mice showed that rabbit hyperimmune sera and recombinant OprF are both able to protect animals from intraperitoneal challenge with P. aeruginosa.
Assuntos
Imunização , Porinas/imunologia , Infecções por Pseudomonas/imunologia , Infecções por Pseudomonas/prevenção & controle , Pseudomonas aeruginosa/imunologia , Animais , Anticorpos Antibacterianos/administração & dosagem , Anticorpos Antibacterianos/sangue , Anticorpos Antibacterianos/farmacologia , Avaliação Pré-Clínica de Medicamentos , Soros Imunes/administração & dosagem , Soros Imunes/farmacologia , Esquemas de Imunização , Imunização Passiva , Injeções Intraperitoneais , Injeções Subcutâneas , Dose Letal Mediana , Camundongos , Testes de Sensibilidade Microbiana , Porinas/metabolismo , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/crescimento & desenvolvimento , Coelhos , Proteínas Recombinantes/imunologiaRESUMO
The cultural, physiologo-biochemical adhesive and antagonistic properties of B. subtilis strains with good prospects for use as biotherapeutic preparations were studied. For further studies B. subtilis strain No. 1719 was chosen. In experiments on non-inbred white mice the animals were treated by the preparation Cifran used for their selective decontamination from opportunistic microflora and for the creation of the state of dysbiosis. The influence of the spore-forming microbe on the parietal microflora of the large intestine of the animals was shown. Reliable data on the changes in the number of microorganisms (CFU/ml) per 1 cm of the surface of the large intestine were established. As markers making it possible to evaluate the action of biotherapeutic and other medicinal remedies, easily determinable ratios of lac+/lac- of bacteria and Staphylococcus aureus/Staphylococcus spp. was proposed.
